CAS, CERN Accelerator School Superconductivity in Particle by S Turner; European Organization for Nuclear Research

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By S Turner; European Organization for Nuclear Research

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Read or Download CAS, CERN Accelerator School Superconductivity in Particle Accelerators: Haus Rissen, Hamburg, Germany, 17-24 May 1995 : proceedings PDF

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Extra info for CAS, CERN Accelerator School Superconductivity in Particle Accelerators: Haus Rissen, Hamburg, Germany, 17-24 May 1995 : proceedings

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J. and Dieckmann, M. (1977). Labelling deoxyribonucleic acid to high specific activity in vivo by nick translation with DNA polymerase I. J. Mol. Biol. 113, 237– 251. Rimsza, L. , Vela, E. , Frutiger, Y. , Rangel, C. , Richter, L. , Grogan, T. M. and Bellamy, W. T. (1996). Rapid automated combined in situ hybridization and immunohistochemistry for sensitive detection of cytomegalovirus in paraffin-embedded tissue biopsies. Am. J. Clin. Pathol. 106, 544–548. Rosenthal, S. , Depowski, P. , Sheehan, C.

K. (1996). Lymphoide changes of the 25 In Situ Hybridization Methods to Study nasopharyngeal and palatine tonsils that are indicative of human immunodeficiency virus infection. A clinicopathologic study of 12 cases. Am. J. Surg. Pathol. 20, 572– 587. Wolber, R. A. and Lloyd, R. V. (1988). Cytomegalovirus detection by nonisotopic in situ DNA hybridization and viral antigen immunostaining using a twocolour technique. Hum. Pathol. 19, 736– 741. Wood, G. S. and Warnke, R. (1981). Suppression of endogenous avidin-binding activity in tissues and its relevance to biotin-avidin detection system.

There was minimal fluorescence of PA-GFP (D), until activated (activated regions outlined) by pulsed 810 nm excitation from a titanium:sapphire laser (E). Transformant expression of AcGFP with a C-terminal signal peptide and N-terminal KDEL ER-retention sequence showed fluorescence within a highly dynamic tubular network (F). The chimeric protein in identical constructs with ZsGreen substituted for AcGFP was targeted to a different subcellular compartment, probably of vacuolar origin (G). The clarity of these structures in a slowly acquired z-series suggests that they were not very mobile.

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