By S Turner; European Organization for Nuclear Research
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Extra info for CAS, CERN Accelerator School Superconductivity in Particle Accelerators: Haus Rissen, Hamburg, Germany, 17-24 May 1995 : proceedings
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There was minimal fluorescence of PA-GFP (D), until activated (activated regions outlined) by pulsed 810 nm excitation from a titanium:sapphire laser (E). Transformant expression of AcGFP with a C-terminal signal peptide and N-terminal KDEL ER-retention sequence showed fluorescence within a highly dynamic tubular network (F). The chimeric protein in identical constructs with ZsGreen substituted for AcGFP was targeted to a different subcellular compartment, probably of vacuolar origin (G). The clarity of these structures in a slowly acquired z-series suggests that they were not very mobile.